pCMV-MEKK1哺乳动物表达载体说明(3)

哺乳动物表达载体说明

can be transfected into mammalian cells using any standard method.

Propagation in E. coli:

Suitable host strains: DH5α, HB101, and other general purpose strains. Single-stranded DNA production requires

a host containing an F plasmid such as JM109 or XL1-Blue.

Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) to E. coli hosts.

E. coli replication origin: pUC Copy number: ≈500

Plasmid incompatibility group: pMB1/ColE1

其他哺乳动物表达载体：  

pCHO1.0 pBApo-CMV-Pur

pcDNA3.1/His C pcDNA5/FRT/V5-His-TOPO pcDNA3.1/His B pcDNA5/FRT/TO-TOPO pcDNA3.1/His A pcDNA5/TO pIRESpuro3 pcDNA5/FRT/TO pIRES2-EGFP pcDNA5/FRT pTT5

pFLAG-CMV2

pNFkB-DD-tdTomato pcDNA3.1/CT-GFP-TOPO pBI-CMV5 pcDNA3.1/NT-GFP-TOPO pSEAP2-Basic pOptiVEC-TOPO pSEAP2-Control pCMV-MEKK1 pBI-CMV3 pCMV-MEK1 pBI-CMV2 pCMV-PKA

pBI-CMV1

pcDNA6.2/nTC-Tag-DEST pNFκB-MetLuc2-Reporter pcDNA6.2/cTC-Tag-DEST pCRE-MetLuc2-Reporter pcDNA3.2/V5/GW/D-TOPO pAcGFP1-Actin

pcDNA6.2/V5/GW/D-TOPO

pAcGFP1-N In-Fusion Ready pcDNA6.2/nGeneBLAzer-GW/D-TOPO pAcGFP1-C3 pcDNA6.2/C-YFP-DEST pAcGFP1-C pcDNA6.2/cGeneBLAzer-DEST pAcGFP1-p53 pcDNA6/V5-His A pAcGFP1-Mito pcDNA6/V5-His B pAcGFP1-Mem pcDNA6/V5-His C pAcGFP1-Lam pcDNA6/myc-His C pAcGFP1-Golgi pcDNA6/myc-His A pAcGFP1-F pcDNA6/myc-His B

pAcGFP1-Hyg-C1 pcDNA6.2/nGeneBLAzer-DEST pAsRed2-N1 pcDNA4/HisMax-TOPO ptdTomato-N1 pcDNA6.2/nLumio-DEST pCMV-tdTomato pcDNA6.2/cLumio-DEST pCRE-DD-tdTomato

pcDNA4/myc-His C

pOPRSVI pREP4 pDual-GC pBK-RSV pBK-CMV pBI-CMV4

pcDNA4/TO/Myc-His/LacZ pOPI3CAT pGene/V5-His B pSwitch pCMVLacI pVgRxR pIND pTRE3G-Luc pTRE3G pTRE2-hygro pTRE-Tight pTK-hyg pTet-On pTet-Off

pTet on advanced pRevTRE pRevTet-On pRevTet-Off pCMV-Tet3G pTRE2 pBD-NF-κB pCMV-AD pCMV-BD pBIND-Id Control pBIND

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