小分子锌指蛋白1在4-硝基喹啉-N-氧化物诱导SD大鼠颊黏膜癌变中的(3)

　　图 4 Western blot检测各组LMO1蛋白的表达

　　Fig 4 Western blot analysis of LMO1 protein expression in the each group

　　上:LMO1蛋白表达的灰度值;下:LMO1蛋白的表达。

　　蛋白标志物作为肿瘤早期筛查的重要指标,积极寻找合适的蛋白标志物对OSCC的早期诊断和判断预后具有重要的指导意义。本实验在保证了病变模型连续性的同时保证了实验的样本量,结果具有可靠性和指导意义。理论上,为探索口腔黏膜癌变的分子机制提供了新思路,丰富了OSCC蛋白标志物的实验研究,为阻断OSCC侵袭转移的途径提供了新的理论依据,成果具有应用前景。但是LMO1的表达变化在OSCC发展进程中的具体作用机制,是否调控EMT进程还尚不明确,这将是后续进一步研究的方向。

　　综上所述,在口腔黏膜癌变模型中,LMO1 mRNA和蛋白异常表达,且mRNA和蛋白表达量与上皮异常增生程度正相关,均随着异常增生程度的增加而表达增加。

　　利益冲突声明:作者声明本文无利益冲突。

　　参考文献

　　[1] Abdalla Z,Walsh T,Thakker N,et al.Loss of epithelial markers is an early event in oral dysplasia and is observed within the safety margin of dysplastic and T1 OSCC biopsies[J].PLoS One,2017,12(12):187-194.

　　[2] 施俊,倪小兵,毛敏,等.Nultin-3联合顺铂对口腔鳞状细胞癌细胞的抑制作用及机制研究[J].实用肿瘤学杂志,2018,32(6):1-10.Shi J,Ni XB,Mao M,et al.Inhibitory effect and mechanism of Nultin-3 combined with cisplatin on oral squamous cell carcinoma[J].Pract Oncol J,2018,32(6):1-10.

　　[3] 张国富,高建明.口腔黏膜白斑和口腔鳞癌中Bcl-2、CDK2AP1的基因表达和临床意义[J].中国现代医学杂志,2017,27(30):56-59.Zhang GF,Gao JM.Expression and clinical value of Bcl-2 and CDK2AP1 in oral leukoplakia and oral squamous carci-noma tissue[J].China J Modern Med,2017,27(30):56-59.

　　[4] Singh S,Gupta V,Vij R,et al.Evaluation of mast cells in oral premalignant and malignant lesions:a histochemical study[J].Nat J Maxillofac Surg,2018,9(2):184-190.

　　[5] Wang C,Niu W,Chen H,et al.Nicotine suppresses apop-tosis by regulating alpha7nAChR/Prx1 axis in oral precan-cerous lesions[J].Oncotarget,2017,8(43):75065-75075.

　　[6] Matthews JM,Lester K,Joseph S,et al.LIM-domain-only proteins in cancer[J].Nat Rev Cancer,2013,13(2):111-122.

　　[7] Sang M,Ma L,Sang M,et al.LIM-domain-only proteins:multifunctional nuclear transcription coregulators that inte-racts with diverse proteins[J].Mol Biol Rep,2014,41(2):1067-1073.

　　[8] Wang XH,Wu HY,Gao J,et al.FGF represses metastasis of neuroblastoma regulated by MYCN and TGF-beta1 in-duced LMO1 via control of let-7 expression[J].Brain Res,2019,17(4):219-228.

　　[9] Chervinsky DS,Zhao XF,Lam DH,et al.Disordered T-cell development and T-cell malignancies in SCL LMO1 double-transgenic mice:parallels with E2A-deficient mice[J].Mol Cell Biol,1999,19(7):5025-5035.

　　[10] 李晓燕.TGF-β诱导的LMO1上调促进食管鳞癌的EMT和转移[D].上海:第二军医大学,2014.Li XY.TGF-β induced upregulation of LMO1 promotes EMT and metastasis of esophageal squamous cell carcinoma[D].Shanghai:Second Military Medical University,2014.

　　[11] 张杨安,关为群,李群,等.口腔鳞状细胞癌组织中Perio-stin的表达及其在上皮间质转化中的作用[J].口腔医学研究,2017,33(3):262-265.Zhang YA,Guan WQ,Li Q,et al.Expression of periostin in oral squamous cell carcinoma and its role in epithehial-mesenchymal transition[J].J Oral Sci Res,2017,33(3):262-265.

　　[12] 孙君君,陶匀雅,周元,等.体外沉默Rce1对舌鳞状细胞癌细胞侵袭和迁移能力的影响[J].华西口腔医学杂志,2019,37(2):143-148.Sun JJ,Tao YY,Zhou Y,et al.Effects of silencing Rce1 in vitro on the invasion and migration of tongue carcinoma[J].West China J Stomotol,2019,37(2):143-148.

　　[13] 罗小波,陈方曼,江宇辰,等.LncRNA ORAOV1-B在口腔鳞癌侵袭转移中的作用研究[C].上海:中华口腔医学会第十次全国口腔黏膜病学术大会暨第八次全国口腔中西医结合学术大会,2018.Luo XB,Chen FM,Jiang YC,et al.The role of LncRNA oraov1-b in the invasion and metastasis of oral squamous cell carcinoma[C].Shanghai:The 10th national conference on oral mucosal diseases and the 8th national conference on integrated traditional Chinese and western medicine of the Chinese association of stomatology.2018.

　　[14] Oldridge DA,Wood AC,Weichert-Leahey N,et al.Genetic predisposition to neuroblastoma mediated by a LMO1 super-enhancer polymorphism[J].Nature,2015,528(7582):418-421.

百度搜索“77cn”或“免费范文网”即可找到本站免费阅读全部范文。收藏本站方便下次阅读，免费范文网，提供经典小说医药类小分子锌指蛋白1在4-硝基喹啉-N-氧化物诱导SD大鼠颊黏膜癌变中的(3)在线全文阅读。