2005The future of animal models of invasive aspergillosis(3)

variabilityandpotentiallyreducesthenumberofanimalsrequiredforstudy[31].

FuturegeneticstudiesusinganimalmodelsThepost-genomiceraofA.fumigatusisextremelyexcitingforthepotentialidentificationofnewdiag-nosticandtherapeutictargetsaswellasfornewinsightsintothepathogenesisandvirulenceoftheorganism.PreliminarystudiesbyZaasetal.[29,32]illustratethepotentialofthesestudies.Theseinvesti-gatorsusedanaerosolchallengeofavarietyofinbredmicetoassesstheroleofhostgeneticbackgroundonsusceptibilitytoAspergillus[32].Interestingly,ingcom-putationalgenetics,achromosomalregionwasthenidentifiedthatcorrelatedwithresistancephenotype.Candidategeneswereidentifiedfromthoseregionandstudiesarecurrentlyongoingtorevealthepotentialsignificanceofthosefindings.Theseresultsdemon-stratethepotentialofcarefullydesignedanimalmodelstoplayanimportantroleinthefutureevaluationintheexperimentalassessmentofinvasivepulmonaryasper-gillosis.

Conclusion

AnimalmodelshavebeenusefulforassessingthepathogenesisandvirulenceofAspergillusandhavesignificantlyaidedthedevelopmentofnewdiagnosticandtherapeuticmodalities.Futuremodelsofexperi-mentalaspergillosisareaimedatassistingintheidentificationanddevelopmentofnewdiagnosticandtherapeutictargetsthatmayresultfromsequencingoftheAspergillusgenome.Thesewillbeofmajorbenefittostudiesaimedultimatelyatimprovingthediagnosisandtreatmentofthisoftenlethalinfection.Acknowledgements

ThisworkhasbeensupportedwithFederalfundsfromtheNationalInstituteofAllergyandInfectiousDis-eases,NationalInstitutesofHealth,underContractNo.N01-AI-30041.

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