限制性内切酶buffer成分及分类

Low-salt buffers

TakaraL L 1 B 10mM Tris-HCl 10mM Tris-HCl 7.5 none 10mM 1mM - MgCl2 DTT 7.5 none 10mM 1mM - MgCl2 DTT Nippon geneNEB10mM 7.0 none 10mM 1mM - Bis-Tris-HCl MgCl2 DTT 10mM Tris-HCl 7.5 none 10mM - MgCl2 8.0 none 10mM - MgCl2 7.5 6mM 6mM NaCl MgCl2 0.1mg/mL BSA - FermentasInvitrogenPromegaREact1 50mM Tris-HCl A 6mM Tris-HCl 1mM - DTT Mid-salt buffers TakaraM M 2 G 10mM Tris-HCl 10mM Tris-HCl 10mM Tris-HCl 10mM Tris-HCl 7.5 50mM NaCl 7.5 50mM NaCl 7.9 50mM NaCl 7.5 50mM NaCl 8.0 50mM NaCl 7.9 50mM NaCl 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM MgCl2 1mM DTT 1mM DTT 1mM DTT - - 1mM DTT - - - 0.1mg/mL BSA - - Nippon geneNEBFermentasInvitrogenPromegaREact2 50mM Tris-HCl C 10mM Tris-HCl High-salt buffers TakaraH H 3 O 50mM Tris-HCl 50mM Tris-HCl 50mM Tris-HCl 50mM 7.5 100mM NaCl 7.5 100mM NaCl 7.9 100mM NaCl 7.5 100mM 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM 1mM - DTT 1mM - DTT 1mM - DTT - 0.1mg/mL Nippon geneNEBFermentasTris-HCl InvitrogenPromegaNaCl 8.0 100mM NaCl 7.5 100mM NaCl MgCl2 10mM MgCl2 6mM MgCl2 - BSA - REact3 50mM Tris-HCl E 6mM Tris-HCl 1mM - DTT KCl buffers TakaraK 20mM Tris-HCl 8.5 100mM KCl 8.5 100mM KCl 7.4 50mM KCl/50mM NaCl 7.5 50mM KCl 10mM MgCl2 10mM MgCl2 6mM MgCl2 7mM MgCl2 1mM DTT - - - 0.1mg/mL BSA - FermentasR 10mM Tris-HCl Invitrogen6 50mM Tris-HCl J 10mM Tris-HCl Promega1mM DTT - Acetic acid buffers TakaraT 33mM Tris-OAc A 20mM Tris-OAc 4 20mM Tris-OAc 7.9 66mM KOAc 7.9 50mM KOAc2 7.9 50mM KOAc 7.9 66mM KOAc 10mM MgOAc2 10mM MgOAc 10mM MgOAc2 10mM MgOAc2 0.5mM DTT 1mM DTT 1mM DTT - - - - 0.1mg/mL Nippon geneNEBFermentasY 33mM Tris-OAc Buffer list of vendors

Takara

Name Property buffer L M H Low salt 10mM Tris-HCl Mid salt 10mM Tris-HCl pH salt 7.5 none 7.5 50mM NaCl 7.5 100mM NaCl metal ion 10mM MgCl2 10mM MgCl2 10mM MgCl2 reducer 1mM DTT 1mM DTT 1mM DTT High salt 50mM Tris-HCl K T KCl salt 20mM Tris-HCl 8.5 100mM KCl 7.9 66mM KOAc 10mM MgCl2 10mM MgOAc2 1mM DTT 0.5mM DTT TrisOAC 33mM Tris-OAc Nippon gene Name Property L M H A B Low salt Mid salt High salt buffer 10mM Tris-HCl 10mM Tris-HCl 50mM Tris-HCl pH salt 7.5 none 7.5 50mM NaCl 7.5 100mM NaCl 7.9 50mM KOAc 8.5 100mM NaCl metal ion 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM MgOAc2 10mM MgCl2 reducer 1mM DTT 1mM DTT 1mM DTT 1mM DTT 1mM DTT Aceticacid 20mM Tris-OAc Basic pH 10mM Tris-HCl NEB Name Property 1 2 3 4 low salt mid salt high salt buffer 10mM Bis-Tris-HCl pH salt 7.0 none metal ion 10mM MgCl2 10mM MgCl2 10mM MgCl2 10mM MgOAc2 reducer 1mM DTT 1mM DTT 1mM DTT 1mM DTT 10mM Tris-HCl 7.9 50mM NaCl 50mM Tris-HCl 7.9 100mM NaCl 7.9 50mM KOAc aceticacid 20mM Tris-OAc Fermentas Name Property buffer B G Blue Green 10mM Tris-HCl 10mM Tris-HCl pH salt 7.5 none 7.5 50mM NaCl metal ion 10mM MgCl2 10mM MgCl2 reducer other - - 0.1mg/mL BSA 0.1mg/mL BSA O R Y Orange Red Yellow 50mM Tris-HCl 10mM Tris-HCl 33mM Tris-OAc 7.5 100mM 10mM NaCl MgCl2 8.5 100mM 10mM KCl MgCl2 7.9 66mM KOAc 10mM MgOAc2 - - - 0.1mg/mL BSA 0.1mg/mL BSA 0.1mg/mL Invitrogen Name Property 1 2 3 4 6 Low salt Mid salt buffer 50mM Tris-HCl 50mM Tris-HCl pH salt 8.0 - 8.0 50mM NaCl metal ion 10mM MgCl2 10mM MgCl2 reducer other - - - - - - - - - - High salt 50mM Tris-HCl KCl 20mM Tris-HCl 8.0 100mM NaCl 10mM MgCl2 7.4 50mM KCl 7.4 50mM KCl/50mM NaCl 8.0 50mM KCl/50mM NaCl 7.4 - 5mM MgCl2 6mM MgCl2 10mM MgCl2 10mM MgCl2 KCl/NaCl 50mM Tris-HCl KCl/NaCl 50mM Tris-HCl Low salt 20mM Tris-HCl 7 - - 8 9 - - - - High salt 100mM Tris-HCl 7.6 150mM NaCl 10mM MgCl2 (*)10mM DTT seperately supplied. Promega

Name Property A B Low salt Mid salt buffer at 37ºC 6mM Tris-HCl 6mM pH salt 7.5 6mM NaCl 7.5 50mM metal ion 6mM MgCl2 6mM reducer other 1mM DTT 1mM - - Tris-HCl C D E F G H J K L NaCl 7.9 50mM NaCl 7.9 150mM NaCl 7.5 100mM NaCl 8.5 100mM NaCl 8.2 - 7.5 50mM NaCl 7.5 50mM KCl 7.4 150mM KCl 9.0 100mM NaCl MgCl2 10mM MgCl2 6mM MgCl2 6mM MgCl2 10mM MgCl2 5mM MgCl2 10mM MgCl2 7mM MgCl2 10mM MgCl2 3mM MgCl2 DTT 1mM DTT 1mM DTT 1mM DTT 1mM DTT - - 1mM DTT - - - - - - - - - - - Mid salt High salt High salt High salt Base pH Strong buffering Mid KCl High KCl Base/High salt 10mM Tris-HCl 6mM Tris-HCl 6mM Tris-HCl 10mM Tris-HCl 50mM Tris-HCl 90mM Tris-HCl 10mM Tris-HCl 10mM Tris-HCl 10mM Tris-HCl

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