质粒提取与酶切电泳实验报告(2)

6 分子实验报告 2013030020华天瑞

Enzymes that cut five or fewer times

Enzyme Recognition frequency Positions __________________________________________________________________________

ApoI r'AATT_y 1 56 BclI T'GATC_A 1 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' 1 36

BsaJI C'CnnG_G 2 36, 37 BstF5I GGATG_nn' 1 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 1 36 FokI GGATGnnnnnnnnn'nnnn_ 1 54 HindIII A'AGCT_T 1 3 Hpy188III TC'nn_GA 1 53 NlaIV GGn'nCC 1 51 SfaNI GCATCnnnnn'nnnn_ 1 32 TaqII CACCCAnnnnnnnnn_nn' 1 28

TspDTI ATGAAnnnnnnnnn_nn' 2 50, 72

Position Enzyme(s)

__________________________________________________________________________

3 HindIII A'AGCT_T 18 BclI T'GATC_A

28 TaqII CACCCAnnnnnnnnn_nn' 32 SfaNI GCATCnnnnn'nnnn_

36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 36 BsaJI C'CnnG_G 37 BsaJI C'CnnG_G 47 BstF5I GGATG_nn'

50 TspDTI ATGAAnnnnnnnnn_nn' 51 NlaIV GGn'nCC

53 Hpy188III TC'nn_GA

54 FokI GGATGnnnnnnnnn'nnnn_ 56 ApoI r'AATT_y

72 TspDTI ATGAAnnnnnnnnn_nn'

Enzymes that do not cut:

_________________________________________________________

AarI, AatII, AccI, Acc65I, AclI, AfeI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI

7 分子实验报告 2013030020华天瑞

AlwI, AlwNI, ApaI, ApaLI, AscI, AseI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BanI

BanII, BbeI, BbsI, BbvI, BbvCI, BceAI, BcgI, BcgI, BciVI, BfrBI, BglI, BglII, BlpI

Bme1580I, BmgBI, BmrI, BmtI, BplI, Bpu10I, BpuEI, BsaI, BsaAI, BsaBI, BsaHI, BsaWI

BsaXI, BsaXI, BseMII, BseRI, BseYI, BsgI, BsiEI, BsiHKAI, BsiWI, BslI, BsmI, BsmAI

BsmBI, BsmFI, Bsp1286I, BspCNI, BspEI, BspHI, BspMI, BsrI, BsrBI, BsrDI, BsrFI BsrGI, BssHII, BssSI, BstAPI, BstBI, BstEII, BstXI, BstYI, BstZ17I, Bsu36I, BtgI

BtsI, Cac8I, ClaI, DraI, DraIII, DrdI, EaeI, EagI, EarI, EciI, Eco57I, EcoICRI EcoNI, EcoO109I, EcoRI, EcoRV, FalI, FauI, FseI, FspI, FspAI, HaeII, HgaI, Hin4I Hin4I, HincII, HpaI, HphI, Hpy8I, HpyF10VI, KasI, KpnI, MboII, MfeI, MluI, MlyI

MmeI, MnlI, MscI, MslI, MspA1I, MwoI, NaeI, NarI, NcoI, NdeI, NgoMIV, NheI, NotI

NruI, NsiI, NspI, PacI, PciI, PflMI, PleI, PmeI, PmlI, PpiI, PpiI, PpuMI, PshAI

PsiI, PspOMI, PsrI, PsrI, PstI, PvuI, PvuII, RsrII, SacI, SacII, SalI, SanDI, SapI SbfI, ScaI, SexAI, SfcI, SfiI, SfoI, SgrAI, SmaI, SmlI, SnaBI, SpeI, SphI, SrfI SspI, StuI, StyI, SwaI, TaqII, TatI, TspGWI, TspRI, Tth111I, XbaI, XcmI, XhoI

XmaI, XmnI, ZraI

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