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山东农业大学学士学位论文

生成的三个文件夹，删除原始安装程序，新建一个用来运行本次比对的文件夹，文件名称中，不能有中文字符。然后将所有“bin”文件夹内的文件，复制到工作目录中，点击电脑界面的“开始”，选择“运行”，输入“cmd”，进入编程编辑界面，进行比对

[17]

。

输入命令如下：

结果如下：

BLASTN 2.2.18 [Mar-02-2008]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), \programs\

Query= Ustilago maydis gapdh (852 letters)

Database: WQ_denove

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山东农业大学学士学位论文

12,006 sequences; 18,561,777 total letters

Searching..................................................done

Score E Sequences producing significant alignments: (bits) Value

GLRG_05186 | Colletotrichum graminicola M1.001 glyceraldehyde-3-... 115 3e-025

>GLRG_05186 | Colletotrichum graminicola M1.001 glyceraldehyde-3-phosphate dehydrogenase (1314 nt) Length = 1314

Score = 115 bits (58), Expect = 3e-025 Identities = 103/118 (87%) Strand = Plus / Plus

Query: 653 actacgttgtcgagtccaccggtgtcttcaccaccattgacaaggcctcggcccacatca 712 Sbjct: 508 actacgtcgtcgagtccactggtgtcttcaccaccaccgacaaggccaaggcccatctta 567

******* *********** **************** ********* ****** * *

Query: 713 agggtggtgccaagaaggtcgtcatctcggctccctcggctgacgcccctatgtacgt 770 Sbjct: 568 agggcggtgccaagaaggtcatcatctctgccccctccgctgacgcccccatgtacgt 625 **** *************** ******* ** ***** *********** ********

Score = 71.9 bits (36), Expect = 4e-012 Identities = 42/44 (95%) Strand = Plus / Plus

Query: 508 tacatgctcaagtacgactccacccacggtgtcttcaacggtga 551 Sbjct: 363 tacatgctcaagtacgactccacccacggcatcttcaacggtga 406 ***************************** *************

BLASTN 2.2.18 [Mar-02-2008]

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山东农业大学学士学位论文

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), \programs\

Query= Botryosphaeria dothidea beta-tubulin gene (432 letters)

Database: WQ_denove

12,006 sequences; 18,561,777 total letters

Searching..................................................done

Score E Sequences producing significant alignments: (bits) Value

GLRG_08786 | Colletotrichum graminicola M1.001 tubulin/FtsZ fami... 172 7e-043 GLRG_11785 | Colletotrichum graminicola M1.001 tubulin/FtsZ fami... 133 6e-031 GLRG_01057 | Colletotrichum graminicola M1.001 tubulin/FtsZ fami... 54 4e-007

>GLRG_08786 | Colletotrichum graminicola M1.001 tubulin/FtsZ family protein (1870 nt) Length = 1870

Score = 172 bits (87), Expect = 7e-043 Identities = 123/135 (91%) Strand = Plus / Plus

Query: 298 caacaagtacgttcctcgtgccgtcctcgtcgacctcgagcccggcacgatggatgccgt 357 Sbjct: 636 caacaagtatgtccctcgcgccgtcctcgtcgacttggagcccggtaccatggatgctgt 695 ********* ** ***** *************** * ******** ** ******** ** Query: 358 ccgcgccggccccttcggccagcttttccgccccgacaacttcgtcttcggtcagtccgg 417 Sbjct: 696 tcgcgccggccccttcggccagcttttccgccccgacaacttcgtctttggccagtccgg 755 *********************************************** ** ********

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山东农业大学学士学位论文

Query: 418 tgccggtaacaactg 432 Sbjct: 756 tgccggcaacaactg 770 ****** ********

Score = 65.9 bits (33), Expect = 1e-010 Identities = 48/53 (90%) Strand = Plus / Plus

Query: 176 tacaatggcacctcggaccttcagctcgagcgcatgaacgtctatttcaacga 228 Sbjct: 503 tacaatggcacctctgagctccagctcgagcgcatgagcgtctacttcaacga 555 ************** ** ** **************** ****** ********

>GLRG_11785 | Colletotrichum graminicola M1.001 tubulin/FtsZ family protein (2024 nt) Length = 2024

Score = 133 bits (67), Expect = 6e-031 Identities = 118/135 (87%) Strand = Plus / Plus

Query: 298 caacaagtacgttcctcgtgccgtcctcgtcgacctcgagcccggcacgatggatgccgt 357 Sbjct: 554 caacaagtacgtcccccgcgccgtcctggtcgatctcgagcccggcaccatggacgccgt 613 ************ ** ** ******** ***** ************** ***** ***** Query: 358 ccgcgccggccccttcggccagcttttccgccccgacaacttcgtcttcggtcagtccgg 417 Sbjct: 614 ccgcgccgggccctacggtcaattgttccgcccggacaacttcgtcttcggtcaggccgg 673 ********* **** *** ** * ******** ********************* **** Query: 418 tgccggtaacaactg 432 Sbjct: 674 cgccggcaacaactg 688 ***** ********

Score = 50.1 bits (25), Expect = 7e-006 Identities = 43/49 (87%) Strand = Plus / Plus

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山东农业大学学士学位论文

Query: 182 ggcacctcggaccttcagctcgagcgcatgaacgtctatttcaacgagg 230 Sbjct: 418 ggcacctccgacatccagctggagcggatgaacgtctacttcaacgagg 466 ******** *** * ***** ***** *********** **********

>GLRG_01057 | Colletotrichum graminicola M1.001 tubulin/FtsZ family protein (1694 nt) Length = 1694

Score = 54.0 bits (27), Expect = 4e-007 Identities = 105/131 (80%) Strand = Plus / Plus

Query: 302 aagtacgttcctcgtgccgtcctcgtcgacctcgagcccggcacgatggatgccgtccgc 361 Sbjct: 475 aagtacgttcctcgtgcagtcctggttgaccttgagcccgcaaccatggatgcaatccgt 534 ***************** ***** ** ***** ******* ** ******** **** Query: 362 gccggccccttcggccagcttttccgccccgacaacttcgtcttcggtcagtccggtgcc 421 Sbjct: 535 tccggtccccttggtgacttcttccgtcccgacaacatggtctacggacagtccggcgcg 594 **** *** * ** * * ***** ********* * **** *** ******** ** Query: 422 ggtaacaactg 432 Sbjct: 595 ggtaacaactg 605 ***********

BLASTN 2.2.18 [Mar-02-2008]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), \programs\

Query= Botryosphaeria dothidea RPB1 (1226 letters)

Database: WQ_denove

12,006 sequences; 18,561,777 total letters

Searching..................................................done

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