禁用偶氮染料释放芳香胺欧盟测试方法,替代原-1&-2方法。
BS EN 14362-1:2012
EN 14362-1:2012 (E)
Annex A (informative)
Chromatographic analyses
A.1 Preliminary remark
As the instrumental equipment of the laboratories may vary (7.8), no generally applicable instructions can be provided for chromatographic analyses. The following parameters have been successfully tested and used.
A.2 Thin layer chromatography (TLC)
A.2.1
Plates (HPTLC):
silica gel 60 with fluorescence indicator F254, (20 × 10) cm;
(2 - 5) µl, applied as a dot;
chloroform/acetic acid (90 + 10) parts per volume.
For NOx-formation, put in an empty chamber a beaker with about 1 ml of sulfuric acid and add a small spatula of solid sodium nitrite. Close the chamber with the lid and let the reaction take place. Put the dry plate in the chamber. After 5 min take it out and dry in a stream of cold air.
Then spray the plate with a solution of 0,2 % α-naphthol prepared in KOH (c= 1 mol/l) in methanol.
1. TLC plates with fluorescence indicator F254
2
Applied volume Mobile solvent 1: Reagent 1:
Reagent 2:
Detection
2. UV lamp and /or after successive treatment with Reagents 1 and 2. Reaction time approximately 5 minutes.
A.2.2
Plates (TLC): Applied volume: Mobile solvent 3: Mobile solvent 4: Development:
--`,,```,,,,````-`-`,,`,,`,`,,`---
silica gel 60, (20 × 10) cm with fluorescence indicator F254; 10,0 µl, applied as a line;
chloroform/ethyl acetate/acetic acid (60 + 30 + 10) parts per volume; chloroform/methanol (95 + 5) parts per volume; n-butyl acetate/toluene (30 + 70) parts per volume; Saturated chamber.
2
16
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